* Televisions incompatible with certain virtual channel and program guide information may use alternative channel numbers.
| Virtual Channel # | RF Channel # | Name | Category |
| 2 | 2 | NBC (WGRZ) | Locals |
| 3 | 3 | MeTV (WBBZ) | Locals |
| 4 | 4 | CBS (WIVB) | Locals |
| 5 | 5 | myTV Buffalo (WNYO) | Locals |
| 6 | 6 | CW23 (WNLO) | Locals |
| 7 | 7 | ABC (WKBW) | Locals |
| 10 | 10 | BLUEview Network | Campus |
| 11 | 11 | Campus TV Station (WNYF) | Campus |
| 12 | 12 | Campus Radio Station (WDVL) | Campus |
| 13 | 13 | FOX (WUTV) | Locals |
| 14 | 14 | FOX Business | News |
| 15 | 15 | CheddarU | News |
| 16 | 16 | CNBC | News |
| 17 | 17 | CNN | News |
| 18 | 18 | CSPAN | News |
| 19 | 19 | CSPAN 2 | News |
| 20 | 20 | Fox News Channel | News |
| 21 | 21 | MSNBC | News |
| 22 | 22 | The Weather Channel | News |
| 23 | 23 | Global Business | International |
| 24 | 24 | CBS Sports Network | Sports |
| 25 | 25 | ESPN | Sports |
| 26 | 26 | ESPN 2 | Sports |
| 27 | 27 | ESPN News | Sports |
| 28 | 28 | ESPNU | Sports |
| 29 | 29 | Fox Sports 1 | Sports |
| 30 | 30 | Golf Channel | Sports |
| 31 | 31 | MAVTV - Motorports Network | Sports |
| 32 | 32 | NFL Network | Sports |
| 33 | 33 | A & E Network | Entertainment |
| 34 | 34 | AMC | Entertainment |
| 35 | 35 | American Heroes Channel | Entertainment |
| 36 | 36 | BBC America | Entertainment |
| 37 | 37 | Comedy Central | Entertainment |
| 38 | 38 | Discovery Channel | Entertainment |
| 39 | 39 | E! Entertainment Television | Entertainment |
| 40 | 40 | FX | Entertainment |
| 41 | 41 | FXX | Entertainment |
| 42 | 42 | History | Entertainment |
| 43 | 43 | Investigation Discovery | Entertainment |
| 44 | 44 | ION Television | Entertainment |
| 45 | 45 | Lifetime | Entertainment |
| 46 | 46 | National Geographic Channel | Entertainment |
| 47 | 47 | OWN | Entertainment |
| 48 | 48 | Reelz Channel | Entertainment |
| 49 | 49 | Science Channel | Entertainment |
| 50 | 50 | Paramount Network | Entertainment |
| 51 | 51 | Syfy | Entertainment |
| 52 | 52 | TBS | Entertainment |
| 53 | 53 | TLC | Entertainment |
| 54 | 54 | TNT | Entertainment |
| 55 | 55 | truTV | Entertainment |
| 56 | 56 | TCM | Entertainment |
| 57 | 57 | USA Network | Entertainment |
| 58 | 58 | We TV | Entertainment |
| 59 | 59 | Animal Planet | Family |
| 60 | 60 | Cartoon Network | Family |
| 61 | 61 | Disney Channel | Family |
| 62 | 62 | Freeform | Family |
| 63 | 63 | GSN | Family |
| 64 | 64 | Hallmark | Family |
| 65 | 65 | Nickelodeon | Family |
| 66 | 66 | TV Land | Family |
| 67 | 67 | Bravo | Lifestyle |
| 68 | 68 | Discovery Life | Lifestyle |
| 69 | 69 | Food Network | Lifestyle |
| 70 | 70 | HGTV | Lifestyle |
| 71 | 71 | LOGO | Lifestyle |
| 72 | 72 | Ovation | Lifestyle |
| 73 | 73 | Pursuit Channel | Lifestyle |
| 74 | 74 | The Travel Channel | Lifestyle |
| 75 | 75 | Viceland | Lifestyle |
| 76 | 76 | AXS TV | Music |
| 77 | 77 | BET | Music |
| 78 | 78 | CMT | Music |
| 79 | 79 | MTV | Music |
| 80 | 80 | MTV2 | Music |
| 81 | 81 | VH1 - Video Hits One | Music |
| 82 | 82 | Showtime | Showtime |
| 83 | 83 | Showtime 2 | Showtime |
| 84 | 84 | Showtime Beyond | Showtime |
| 85 | 85 | Showtime Extreme | Showtime |
| 86 | 86 | Showtime Next | Showtime |